Inhibition by somatostatin interneurons in olfactory cortex
AM Large, NA Kunz, S Mielo and A-MM Oswald
Frontiers Neural Circuits 17 August 2016, http://dx.doi.org/10.3389/fncir.2016.00062
In this study we characterized the spatial distributions of somatostatin and parvalbumin expressing interneurons in anterior piriform cortex in SST-cre, GIN, PV-cre and G42 mouse lines. The interneuron distributions were similar to previous immunochemistry studies in SST-cre and PV-cre lines. However, unlike neocortical areas, labeled interneurons in the GIN (SST) and G42 (PV) lines were nearly absent in piriform cortex. Next we characterized the electrophysiological properties of SST interneurons and found a large proportion were fast-spiking and very similar to PV interneurons. When we optogenetically activated SST-cells, we recorded IPSCs in the majority of L3 interneurons including SST-cells. PV cells appear to receive the strongest inhibition of the interneuron classes. Finally we show that pyramidal cells receive both dendritic and perisomatic inhibition from SST-cells.
AM Large, NA Kunz, S Mielo and A-MM Oswald
Frontiers Neural Circuits 17 August 2016, http://dx.doi.org/10.3389/fncir.2016.00062
In this study we characterized the spatial distributions of somatostatin and parvalbumin expressing interneurons in anterior piriform cortex in SST-cre, GIN, PV-cre and G42 mouse lines. The interneuron distributions were similar to previous immunochemistry studies in SST-cre and PV-cre lines. However, unlike neocortical areas, labeled interneurons in the GIN (SST) and G42 (PV) lines were nearly absent in piriform cortex. Next we characterized the electrophysiological properties of SST interneurons and found a large proportion were fast-spiking and very similar to PV interneurons. When we optogenetically activated SST-cells, we recorded IPSCs in the majority of L3 interneurons including SST-cells. PV cells appear to receive the strongest inhibition of the interneuron classes. Finally we show that pyramidal cells receive both dendritic and perisomatic inhibition from SST-cells.